Effects of nano-selenium on the liver antioxidant enzyme activity and immunoglobolins in male rats exposed to oxidative stress

The aim of this study was to evaluate the effects of nano-particle of selenium on liver antioxidant activity, blood cells count and IgG level in rats exposed to oxidative stress. Twenty four male rats were randomly divided into four treatments with six replicates. Treatments included of: control group gavaged and injected (ip) normal saline; T1: injected (ip) tert butyl hydroperoxide (t-BHP) at 0.2 mmol per kg body weight and gavaged normal saline; T2: gavaged nano selenium (0.3 mg per kg body weight) and injected (ip) normal saline, and T3: injected (ip) t-BHP (0.2 mmol per kg body weight) following gavaged nano-Selenium (0.3 mg per kg body weight). Experimental period was lasted for 35 days and administrations were done each 48 hour. Oxidative stress (T2) increased the liver activities of antioxidant enzymes and also the levels of liver enzymes released in the plasma as compared with other treatments. T3 had lower (P <0.05) enzyme activities than T2 group. T2 had the lowest (P<0.05) and T1 had the highest red blood cells, white blood cells, neutrophil and lymphocyte counts. T3 had higher count of red blood cells than T2. Gavage of nano-selenium in stressed rats had no effect on total and differential white cells count compared with stressed rats (T2) or the control group. In stressed rats (T2 and T3), IgG was decreased compared to non-stressed rats. It was concluded that nanoselenium supplementation ameliorate the negative effects of oxidative stress on liver and influence positively in red blood cells count and immunoglobulin production.